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Summary

"The best feature of this lab manual is its style and organization. It is not just readable, it is thorough.... It is comparable to the best lab manuals I have used or read." - William Coleman, University of Hartford "It is a friendly-to-student book and I think student users will appreciate it--even if they don\'t realize why. I think they will like microbiology just a bit more." -Loyd J. Hays, University of Texas at San Antonio Clear, concise, and always engaging! Written specifically for allied health students, Pollack, Findlay, Mondschein, and Modesto's Laboratory Exercises in Microbiology, 2/e focuses on what students need to succeed in the lab: clear, concise, and highly engaging exercises and activities, rather than content that is simply adapted from a standard microbiology textbook. Throughout the manual, a variety of interactive activities and experiments convey the basic concepts of microbiology--all in a student-friendly tone. Students also have the opportunity to explore methods that allow the safe movement or transfer of microbial cells from one type of growth environment to another, classification and identification of microbes, microbial biochemistry, as well as laboratory exercises from medical, food, and environmental microbiology.

Table of Contents

Preface iii
Introduction: Laboratory Operations and Safety: Hand Washing vii
PART I GENERAL MICROSCOPY AND ASEPTIC TECHNIQUE
1(30)
Laboratory Safety: Introduction to the Microscope
3(14)
Transfer and Isolation Techniques
17(14)
Microbes in the Environment
25(6)
PART II MICROBIAL MORPHOLOGY, DIFFERENTIAL STAINS
31(60)
Cultural and Cellular Morphology
33(10)
Bacterial Growth
43(8)
Gram Stain
51(8)
Acid-Fast Stain
53(6)
Endospore Stain
59(8)
Capsule Stain
61(1)
Hanging Drop Technique
62(5)
Fungi
67(8)
Viruses---Visualization and Enumeration
75(6)
Parasitology
81(10)
PART III MICROBIAL CONTROL AND BIOCHEMISTRY
91(42)
Microbial Sensitivity Testing: Ultraviolet Light Sensitivity
93(14)
Heat Sensitivity
96(2)
Chemical Sensitivity
98(2)
Antibiotic Sensitivity: Kirby-Bauer Technique
100(7)
Bacterial Biochemistry
107(10)
Gas Requirements of Microorganisms
117(6)
Specialized Media
123(10)
PART IV MEDICAL MICROBIOLOGY
133(78)
Genetics
135(6)
Epidemiology
141(8)
Speciman-Handling Protocols
149(12)
Specific Laboratory Tests
161(8)
Serology
169(10)
Identification of Enteric Pathogens: Traditional Methods
179(8)
Identification of Enteric Pathogens: Rapid Identification Methods
187(10)
Identification of a Bacterial Unknown: The Gram-Negative Unknown
197(8)
Identification of a Bacterial Unknown: The Gram-Positive Cocci
205(6)
PART V FOOD AND ENVIRONMENTAL MICROBIOLOGY
211(32)
Identification and Quantification of Microbial Numbers in a Water Sample
213(6)
Identification of Microbes in Beef and Poultry and the Quantification of Microbial Numbers
219(6)
Soil Microbiology
225(6)
Photographic Atlas
1(230)
Microbial Ecology
231(6)
Biofilms
237(6)
Bibliography 243(2)
Appendix 1 245(5)
Appendix 2 250(1)
Appendix 3 251(1)
Credits 252(1)
Index 253

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